About the Project

The RAID project arose as a result of work conducted during late 2022 in preparation for the first study in LEV Foundation's Robust Mouse Rejuvenation (RMR) program: specifically, a comprehensive survey conducted by LEVF's Chief Science Monitor, Maximus Peto, of publications documenting successful lifespan extension in strains of rodents (mice and rats) with normal baseline lifespans. That survey played an important role in informing the selection of interventions for RMR-1.

Recognising that this compilation of data may be of interest to other researchers in the field of longevity/aging research, we decided to make it publicly available. To enable convenient exploration of the results, we have also developed a visualization tool which depicts the increases in lifespan achieved in different studies as a single bar chart.

The dataset queried by this tool is intended to cover all published studies that meet the inclusion criteria:

• Species: Mice (Mus musculus) or rats (Rattus norvegicus);
• Genetic background consistent with normal aging rates (e.g. no progeria, PolG (mtDNA) mutator, Alzheimer's models such as APP/PS1, etc.);
• Reports an intervention with some statistically significant effect on average or maximum lifespan.

It is our intention to update the dataset periodically, but some newer (or recently identified) publications may not yet be indexed; please see below for a list of currently-known exceptions.

Studies Pending Inclusion

• Ribosomal protein S6 kinase 1 signaling regulates mammalian life span. Science. 2009 Oct 2;326(5949):140-4.
• Rapamycin-induced insulin resistance is mediated by mTORC2 loss and uncoupled from longevity. Science. 2012 Mar 30;335(6076):1638-43.
• Increased mammalian lifespan and a segmental and tissue-specific slowing of aging after genetic reduction of mTOR expression. Cell Rep. 2013 Sep 12;4(5):913-20.
• Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan. Nature. 2016 Feb 11;530(7589):184-9.
• Canagliflozin extends life span in genetically heterogeneous male but not female mice. JCI Insight. 2020 Nov 5;5(21):e140019.
• Body temperature is a more important modulator of lifespan than metabolic rate in two small mammals. Nat Metab. 2022 Mar;4(3):320-326.
• In vivo cyclic induction of the FOXM1 transcription factor delays natural and progeroid aging phenotypes and extends healthspan. Nat Aging. 2022 May;2(5):397-411.
• Lifespan extension in female mice by early, transient exposure to adult female olfactory cues. Elife. 2022 Dec 16;11:e84060.
• Dietary supplementation of clinically utilized PI3K p110α inhibitor extends the lifespan of male and female mice. Nat Aging. 2023 Feb;3(2):162-172.
• Hypothalamic Menin regulates systemic aging and cognitive decline. PLoS Biol. 2023 Mar 16;21(3):e3002033.
• The effect of a pharmaceutical ghrelin agonist on lifespan in C57BL/6J male mice: A controlled experiment. Aging Cell. 2023 Apr;22(4):e13787.
• Rapamycin treatment increases survival, autophagy biomarkers and expression of the anti-aging klotho protein in elderly mice. Pharmacol Res Perspect. 2023 Jun;11(3):e01091.
• Taurine deficiency as a driver of aging. Science. 2023 Jun 9;380(6649):eabn9257.
• Prepubertal castration eliminates sex differences in lifespan and growth trajectories in genetically heterogeneous mice. Aging Cell. 2023 Aug;22(8):e13891.
• CD133⁺ endothelial-like stem cells restore neovascularization and promote longevity in progeroid and naturally aged mice. Nat Aging. 2023 Nov;3(11):1401-1414.
• Dietary restriction of isoleucine increases healthspan and lifespan of genetically heterogeneous mice. Cell Metab. 2023 Nov 7;35(11):1976-1995.e6.
• Astaxanthin and meclizine extend lifespan in UM-HET3 male mice; fisetin, SG1002 (hydrogen sulfide donor), dimethyl fumarate, mycophenolic acid, and 4-phenylbutyrate do not significantly affect lifespan in either sex at the doses and schedules used. Geroscience. 2023 Dec 2.
• DMH^Ppp1r17 neurons regulate aging and lifespan in mice through hypothalamic-adipose inter-tissue communication. Cell Metab. 2024 Jan 1:S1550-4131(23)00462-X.
• Mucosal TLR5 activation controls healthspan and longevity. Nat Commun. 2024 Jan 2;15(1):46.

If you have any queries regarding the project, or wish to alert us to a study that meets the inclusion criteria but which is not yet indexed nor listed above in the "Studies Pending Inclusion" section, please write to raid@levf.org.

Query Development, Data Compilation and Curation: Maximus Peto
Software Tool Development: Tony Floyd
Hosting/Styling: Ben Zealley
Funding: Longevity Escape Velocity (LEV) Foundation & Long Life Labs, LLC.