About the Project

A concise video tutorial covering the RAID project's goals and the functionality of the web tool is available to watch now via LEVF's YouTube channel.

The RAID project arose as a result of work conducted during late 2022 in preparation for the first study in LEV Foundation's Robust Mouse Rejuvenation (RMR) program: specifically, a comprehensive survey conducted by LEVF's Chief Science Monitor, Maximus Peto, of publications documenting successful lifespan extension in strains of rodents (mice and rats) with normal baseline lifespans. That survey played an important role in informing the selection of interventions for RMR-1.

Recognising that this compilation of data may be of interest to other researchers in the field of longevity/aging research, we decided to make it publicly available. To enable convenient exploration of the results, we have also developed a visualization tool which depicts the increases in lifespan achieved in different studies as a single bar chart.

A peer-reviewed report on the project's architecture and methodology was published in Aging (Albany NY) in March 2025, and is free to view online.

The dataset queried by this tool is intended to cover all published studies that meet the inclusion criteria:

• Species: Mice (Mus musculus) or rats (Rattus norvegicus);
• Genetic background consistent with normal aging rates (e.g. no progeria, PolG (mtDNA) mutator, Alzheimer's models such as APP/PS1, etc.);
• Reports an intervention with some statistically significant effect on average or maximum lifespan.

It is our intention to update the dataset periodically, but some newer (or recently identified) publications may not yet be indexed; please see below for a list of currently-known exceptions.

As of December 2025, our collection included 183 published studies.

Studies Pending Inclusion

• Increase in lifespan of rats following polypeptide pineal extract treatment. Exp Pathol (Jena). 1979;17(9):539-45.
• Carcinogenesis and aging. IV. Effect of low-molecular-weight factors of thymus, pineal gland and anterior hypothalamus on immunity, tumor incidence and life span of C3H/Sn mice. Mech Ageing Dev. 1982 Jul;19(3):245-58.
• Extension of lifespan in mice treated with Dinh lang (Policias fruticosum L.) and (-)deprenyl. Acta Physiol Hung. 1992;79(2):119-24.
• Low methionine ingestion by rats extends life span. J Nutr. 1993 Feb;123(2):269-74.
• Longevity effect of chromium picolinate--'rejuvenation' of hypothalamic function? Med Hypotheses. 1994 Oct;43(4):253-65.
• The p66shc adaptor protein controls oxidative stress response and life span in mammals. Nature. 1999 Nov 18;402(6759):309-13.
• The Snell dwarf mutation Pit1(dw) can increase life span in mice. Mech Ageing Dev. 2002 Jan;123(2-3):121-30.
• Coenzyme Q supplementation protects from age-related DNA double-strand breaks and increases lifespan in rats fed on a PUFA-rich diet. Exp Gerontol. 2004 Feb;39(2):189-94.
• Evolutionary conservation of the clk-1-dependent mechanism of longevity: loss of mclk1 increases cellular fitness and lifespan in mice. Genes Dev. 2005 Oct 15;19(20):2424-34.
• Long-term modulation of electrical synapses in the mammalian thalamus. Science. 2005 Dec 16;310(5755):1809-13.
• Targeted disruption of growth hormone receptor interferes with the beneficial actions of calorie restriction. Proc Natl Acad Sci U S A. 2006 May 16;103(20):7901-5.
• Transgenic mice with a reduced core body temperature have an increased life span. Science. 2006 Nov 3;314(5800):825-8.
• Delayed ageing through damage protection by the Arf/p53 pathway. Nature. 2007 Jul 19;448(7151):375-9.
• Genetic suppression of GH-IGF-1 activity, combined with lifelong caloric restriction, prevents age-related renal damage and prolongs the life span in rats. Am J Nephrol. 2008;28(5):755-64.
• Insulin sensitivity as a key mediator of growth hormone actions on longevity. J Gerontol A Biol Sci Med Sci. 2009 May;64(5):516-21.
• Macrophage migration inhibitory factor-knockout mice are long lived and respond to caloric restriction. FASEB J. 2010 Jul;24(7):2436-42.
• Deficiency of the lipid synthesis enzyme, DGAT1, extends longevity in mice. Aging (Albany NY). 2012 Jan;4(1):13-27.
• Acarbose, 17-α-estradiol, and nordihydroguaiaretic acid extend mouse lifespan preferentially in males. Aging Cell. 2014 Apr;13(2):273-82.
• Combined statin and angiotensin-converting enzyme (ACE) inhibitor treatment increases the lifespan of long-lived F1 male mice. Age (Dordr). 2016 Dec;38(5-6):379-391.
• A Long-lived Mouse Lacking Both Growth Hormone and Growth Hormone Receptor: A New Animal Model for Aging Studies. J Gerontol A Biol Sci Med Sci. 2017 Aug 1;72(8):1054-1061.
• Hypothalamic stem cells control ageing speed partly through exosomal miRNAs. Nature. 2017 Aug 3;548(7665):52-57.
• Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice. BMC Microbiol. 2019 Jun 13;19(1):130.
• Lifespan of long-lived growth hormone receptor knockout mice was not normalized by housing at 30°C since weaning. Aging Cell. 2020 May;19(5):e13123.
• Long-term treatment with spermidine increases health span of middle-aged Sprague-Dawley male rats. Geroscience. 2020 Jun;42(3):937-949.
• Sodium rutin extends lifespan and health span in mice including positive impacts on liver health. Br J Pharmacol. 2022 May;179(9):1825-1838.
• MyMD-1 Improves Health Span and Prolongs Life Span in Old Mice: A Noninferiority Study to Rapamycin. J Gerontol A Biol Sci Med Sci. 2023 Feb 24;78(2):227-235.
• Increased hyaluronan by naked mole-rat Has2 improves healthspan in mice. Nature. 2023 Sep;621(7977):196-205.
• Fasting mimicking diet extends lifespan and improves intestinal and cognitive health. Food Funct. 2024 Apr 22;15(8):4503-4514.
• Longevity extension in rats via improved redox homeostasis with high carbohydrate diet intervention from weaning to adulthood: a comprehensive multi-omics study. Food Funct. 2024 Jul 29;15(15):7920-7935.
• Long-term effects of s-KL treatment in wild-type mice: Enhancing longevity, physical well-being, and neurological resilience. Mol Ther. 2025 Apr 2;33(4):1449-1465.
• The lifespan-extending MEK1 inhibitor trametinib promotes regulation of de novo lipogenesis enzymes by chaperone-mediated autophagy. Front Aging. 2025 Jun 25;6:1621808.
• Psilocybin treatment extends cellular lifespan and improves survival of aged mice. NPJ Aging. 2025 Jul 8;11(1):55.
• Sex-specific longitudinal reversal of aging in old frail mice. Aging (Albany NY). 2025 Aug 21;17(9):2252-2277.
• CD38-Targeting Peptide Vaccine Ameliorates Aging-Associated Phenotypes in Mice. Aging Cell. 2025 Sep;24(9):e70147.
• Extension of lifespan by epicatechin, halofuginone and mitoglitazone in male but not female genetically heterogeneous mice. Geroscience. 2025 Sep 19.
• The protein phosphatase 6 subunit SAPS3 modulates lifespan by regulating metabolism. Sci Adv. 2025 Oct 24;11(43):eadt3879.
• Glycation-lowering compounds inhibit ghrelin signaling to reduce food intake, lower insulin resistance, and extend lifespan. Cell Rep. 2025 Oct 28;44(10):116422.
• FOXO3a upregulates DNA repair activities by transcriptional activation of target genes and provides the resistance to gamma radiation and the extension of lifespan in mouse. Biogerontology. 2025 Oct 28;26(6):196.
• Elastin-derived extracellular matrix fragments drive aging through innate immune activation. Nat Aging. 2025 Dec;5(12):2380-2398.
• Sex-Specific transcriptomic changes in adipose tissue following adult-onset disruption of growth hormone receptor. Pituitary. 2025 Dec 5;29(1):5.
• Mitochondrial Respiratory Supercomplex Assembly Factor COX7RP Contributes to Lifespan Extension in Mice. Aging Cell. 2026 Jan;25(1):e70294.

If you have any queries regarding the project, or wish to alert us to a study that meets the inclusion criteria but which is not yet indexed nor listed above in the "Studies Pending Inclusion" section, please write to raid@levf.org.

Query Development, Data Compilation and Curation: Maximus Peto
Software Tool Development: Tony Floyd
Hosting/Styling: Ben Zealley
Funding: Longevity Escape Velocity (LEV) Foundation & Long Life Labs, LLC.

We are grateful to the numerous contributors who have identified additional studies to include in RAID, particularly the very industrious Florin Clapa.